Sartorius Stedim
BioTech Instrumentation
Sartorius Stedim Biotech – Virus Counter® 3100
Fluidics System Developer
Optoelectronic Integration Lead
Calibration & Validation Engineer
Software–Hardware Interface Collaborator
Reliability & Test Contributor
Project Overview / MIssion Objectives
The Virus Counter® 3100 is a purpose-built analytical platform that enables rapid, direct quantification of total virus particles in bioprocess samples. Utilizing a no-wash, fluorescence-based assay, the system delivers results in minutes, offering near real-time insights into viral titers. This capability is crucial for optimizing viral vector production, vaccine development, and gene therapy manufacturing processes.
The platform integrates advanced fluidics, optics, and software to detect and measure virus particles with high specificity and sensitivity, streamlining workflows and enhancing product quality in biopharmaceutical applications.
Roles and Responsibilities
As a key member of the engineering team, I contributed to the development of the Virus Counter® 3100, focusing on:
Designing and optimizing the fluidic systems to ensure precise sample handling and consistent flow rates through the optical detection zone.
Collaborating with cross-functional teams to integrate optical components, including lasers and photomultiplier tubes, for accurate fluorescence detection.
Developing calibration protocols and validation procedures to ensure compliance with regulatory standards and maintain measurement accuracy.
Implementing software algorithms for real-time data analysis and user-friendly interface, facilitating ease of operation and data interpretation.
Conducting rigorous testing and troubleshooting to refine system performance and reliability under various operational conditions.
Legacy
The Virus Counter® 3100 has become an essential tool in the biopharmaceutical industry, enabling faster decision-making and improved control over viral production processes. Its adoption has led to increased efficiency in vaccine and gene therapy development, contributing to the advancement of critical healthcare solutions.
My contributions to this project have reinforced the importance of interdisciplinary collaboration and precision engineering in developing instruments that have a tangible impact on public health.
Highlight: Integrating Advanced Detection Technologies
A significant aspect of the Virus Counter® 3100’s innovation lies in its integration of advanced detection technologies:
Fluorescence-Based Assays: Utilizing ViroTag® reagents, the system employs a no-wash protocol to label virus particles, enabling rapid and specific detection.
Optical Detection System: The instrument’s optical setup, including lasers and photomultiplier tubes, allows for sensitive detection of fluorescent signals, ensuring accurate quantification of virus particles.
Real-Time Data Analysis: Integrated software processes the detected signals in real-time, providing immediate feedback on viral titers and facilitating prompt decision-making in bioprocess workflows.
These technological advancements collectively contribute to the system’s ability to deliver precise, rapid, and reliable virus quantification, addressing a critical need in biopharmaceutical manufacturing.